5 research outputs found
Ladderane phospholipids inanammox bacteria comprise phosphocholine and phosphoethanolamine headgroups
Anammox bacteria present in wastewater treatment systems and marine environments
are capable of anaerobically oxidizing ammonium to dinitrogen gas. This
anammox metabolism takes place in the anammoxosome which membrane is
composed of lipids with peculiar staircase-like ‘ladderane’ hydrocarbon chains that
comprise three or four linearly concatenated cyclobutane structures. Here, we
applied high-performance liquid chromatography coupled to electrospray ionization
tandem mass spectrometry and matrix-assisted laser desorption/ionization
time-of-flight mass spectrometry to elucidate the full identity of these ladderane
lipids. This revealed a wide variety of ladderane lipid species with either a
phosphocholine or phosphoethanolamine polar headgroup attached to the
glycerol backbone. In addition, in silico analysis of genome data gained insight
into the machinery for the biosynthesis of the phosphocholine and phosphoethanolamine
phospholipids in anammox bacteria
Ladderane phospholipids in anammox bacteria comprise phosphocholine and phosphoethanolamine headgroups
Contains fulltext :
35723.pdf (publisher's version ) (Closed access